Characterization and Isolation of Bio-Functional Lipids from Black Sea Urchin Stomopneustes variolaris and its Multi Potential In-vitro Activities

Abstract

Lipids and lipid fractions isolated from gonads of Stomopneustes variolaris was demonstrated for its bio-functional effects like antioxidant and antimicrobial activity. Gas Chromatography- Mass Spectrometry (GC-MS) analysis detected hexatriacontane (17.03%) as the major compound in crude lipid sample and Phenol, 2,4-bis (1, 1-dimethylethyl) (83.50%) showed high retention peak area in the GC-MS chromatogram of all the three lipid fractions. FTIR spectra confirmed that the esters are the predominant group in the lipid fraction. 1H NMR evaluation spotted peaks of methanol and aromatic groups. Lipid fractions showed bactericidal effect against two Gram positive bacteria (Streptococcus aureus, Pseudomonas aeruginosa), three gram negative bacteria (Escherichia coli, Klebsiella pneumoniae, Vibrio cholera) and fungicidal effect against the fungal species (Candida albicans) tested. In-vitro antioxidant assays, such as lipid peroxidation and Diphenyl picrylhydrazyl (DPPH) assays also showed positive results towards scavenging free radicals. Hence, bio lipids from Stomopneustes variolaris could be further explored towards the development of new bio-therapeutic formulations.