Abstract
Bacteriophage PAXYB1 was recently isolated from wastewater samples. This phage was chosen based on its lytic properties against clinical isolates of Pseudomonas aeruginosa (P. aeruginosa). In the present study, characterized PAXYB1, clarified its morphological and lytic properties, and analyzed its complete genome sequence. Based on the morphology of PAXYB1, it is a Podoviridae. The linear GC-rich (62.29%) double-stranded DNA genome of PAXYB1 is 43,337 bp including direct terminal repeats (DTRs) of 468 bp. It contains 60 open reading frames (ORFs) that are all encoded within the same strand. We also showed that PAXYB1 is a virulent phage and a new member of the phiKMV-like phages genus. Twenty-eight out of sixty predicted gene products (gps) showed significant homology to proteins of known function, which were confirmed by analyzing the structural proteome. Altogether, our work identified a novel lytic bacteriophage that lyses P. aeruginosa PAO1 and efficiently infects and kills several clinical isolates of P. aeruginosa. This phage has potential for development as a biological disinfectant to control P. aeruginosa infections.
Highlights
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic human pathogen that is widely distributed in various environments
The lytic phage PAXYB1 isolated from wastewater was used to infect P. aeruginosa strain PAO1
The results showed that PAXYB1 was able to infect 13 of the 20 tested strains and formed clear plaques on 10 of the strains (Table 1), indicating that PAXYB1 can efficiently infect and kill some clinical isolates of P. aeruginosa
Summary
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic human pathogen that is widely distributed in various environments. Phage therapy has been used for the control and treatment of multidrug-resistant P. aeruginosa in lung infections in mouse models and in cystic fibrosis airway lung cells in vitro[7,8]. The isolation of new phages against non-laboratory strains of P. aeruginosa and an analysis of their genomes and lytic behaviour will lead to a more comprehensive view of the antimicrobial potential of virulent P. aeruginosa phages. We isolated and characterized a new phage infecting P. aeruginosa named PAXYB1. It is a Podoviridae from its virion structure, and it has a strong ability to infect several clinical isolates of P. aeruginosa. In-depth characterization of this new phage will contribute to the promising therapeutic use of this phage
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