Abstract
In this study, a novel Escherichia coli-specific bacteriophage, vB_EcoM_IME392, was isolated from chicken farm sewage in Qingdao, China. The genome of IME392 was found by next-generation sequencing to be 116,460 base pairs in length with a G+C content of 45.4% (GenBank accession number MH719082). BLASTn results revealed that only 2% of the genome sequence of IME392 shows sequence similarity to known phage sequences in the GenBank database, which indicates that IME392 is a novel bacteriophage. Transmission electron microscopy showed that IME392 belongs to the family Myoviridae. The host range, the multiplicity of infection, and a one-step growth curve were also determined.
Highlights
Since the German pediatrician Theodor Escherich isolated E. coli from healthy human feces in 1885, it has been extensively and thoroughly studied [7, 8, 21, 22, 24]
Transmission electron microscopy results suggest that bacteriophage IME392 is morphologically classified as Myoviridae, possessing an icosahedral head of approximately 83.93 ± 0.55 nm in diameter (n=10) and a contractile tail of 122.23 ± 3.55 nm in length (n=10) (Fig. 1)
At the same time, the problem of drug resistance and even multi-drug resistance caused by the antibiotics abuse has brought new challenges to mankind
Summary
Since the German pediatrician Theodor Escherich isolated E. coli from healthy human feces in 1885, it has been extensively and thoroughly studied [7, 8, 21, 22, 24]. Escherichia coli, as a model organism, currently plays a vital role in life science research and biotechnology industries such as pharmaceuticals and industrial chemicals [5, 13, 18]. E. coli is an important microorganism ubiquitous in the natural environment and mammalian gastrointestinal tract, and is part of the normal intestinal flora. Most E. coli are harmless, but certain serotypes can cause severe food poisoning, septic shock, meningitis, or urinary tract infections [16, 29], which seriously threaten human life and property safety. There have been reports of strains resistant to all major antibiotic types in E. coli, including extended-spectrum-beta-lactams, carbapenems, fluoroquinolones, aminoglycosides, trimethoprim-sulfamethoxazole [28]. Phage therapy has certain advantages over antibiotic therapy: low cost, easy availability, specificity, and low side effects
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