Abstract
Probiotics can modulate the composition of gut microbiota and benefit the host animal health in multiple ways. Lactic acid bacteria (LAB), mainly Lactobacillus and Bifidobacterium species, are well-known microbes with probiotic potential. In the present study, 88 microbial strains were isolated from canine feces and annotated. Among these, the four strains CACC517, 537, 558, and 566 were tested for probiotic characteristics, and their beneficial effects on hosts were evaluated both in vitro and in vivo; these strains exhibited antibiosis, antibiotic activity, acid and bile tolerance, and relative cell adhesion to the HT-29 monolayer cell line. Byproducts of these strains increased the viability and decreased oxidative stress in mouse and dog cell lines (RAW264.7 and DH82, respectively). Subsequently, when the probiotics were applied to the clinical trial, changes in microbial composition and relative abundance of bacterial strains were clearly observed in the experimental animals. Experimental groups before and after the application were obviously separated from PCA analysis of clinical results. Conclusively, these results could provide comprehensive understanding of the effects of probiotic strains (CACC517, 537, 558, and 566) and their industrial applications.
Highlights
According to the 2001 definition by the World Health Organization (WHO), probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host (Hotel and Cordoba, 2001)
A total of 88 bacterial strains were isolated and probiotic candidates were selected through prescreening
Genomic Structure and Genetic Feature Whole genome sequences of the strains CACC517, CACC537, CACC558, and CACC566 were uploaded to NCBI with accession IDs PRJNA599536, PRJNA601629, PRJNA601672, and PRJNA601660, respectively
Summary
According to the 2001 definition by the World Health Organization (WHO), probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host (Hotel and Cordoba, 2001). In the interaction between host and probiotics, the mechanisms are generally categorized as act on competition between probiotics and pathogenic organisms for an adhesion site or a nutrient compound, synthesis of antimicrobial compounds by probiotics, and modulation of the host immune system. These modes of action are considered when screening novel probiotic strains (Shokryazdan et al, 2017; Singh et al, 2018). The findings of this study can contribute to the establishment of an integrated model for characterizing novel probiotics, and the characterized probiotics may have potential for use in industrial fields related to dogs
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