Abstract
A cDNA encoding NADP-dependent malic enzyme was isolated from castor bean (Ricinus communis L.) endosperm. The 2362-bp cDNA encodes a 641 amino acid protein, which is predicted to have a 50 amino acid chloroplast transit peptide. Southern blotting revealed a single copy of the gene. Recombinant protein expression confirmed that the cDNA encodes a NADP-dependent malic enzyme; both Western blotting and enzyme assays detected NADP-dependent malic enzyme. Removal of the transit peptide in the recombinant protein did not affect the pH optimum of the enzyme, but it did result in an increased specific activity.Key words: Ricinus communis, NADP-dependent malic enzyme (NADP-ME), fatty acid biosynthesis, leucoplast, recombinant protein expression, transit peptide.
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