Abstract
Rice blast is a destructive disease caused by Magnaporthe oryzae, and it has a large impact on rice production worldwide. Compared with leaf blast resistance, our understanding of panicle blast resistance is limited. The japonica landrace Jiangnanwan from Taihu Lake region in China shows highly resistance to panicle and leaf blast. In this study, three generations (F2:5, F2:6, F2:7) consisting of 221 RILs (recombination inbreeding lines), developed from the cross of Jiangnanwan and Suyunuo, a susceptible-blast japonica variety, were evaluated for panicle blast resistance in the fields and leaf blast resistance in greenhouse in Nanjing in 2013, 2014 and 2015. A blast resistance gene Pi-jnw1 referring to panicle blast resistance and leaf blast resistance was identified in the three generations and located in the region of RM27273 and RM27381 in chromosome 11. The RIL18 line harboring Pi-jnw1 was selected to be backcrossed with Suyunuo to develop BC2F2 populations. According to the genotyping of 1,150 BC2F2 individuals and panicle blast and leaf blast resistance evaluation of 47 recombinants between RM27150 and RM27381, Pi-jnw1 was finally mapped to the 282 kb region between markers W28 and BS39. This study revealed that Jiangnanwan harboring a panicle blast and leaf blast resistance gene Pi-jnw1 could be a genetic source for breeding new rice cultivars with panicle blast resistance.
Highlights
In 2013, 2014 and 2015, Jiangnanwan and Suyunuo were inoculated with the strain Hoku1 in field for panicle blast resistance evaluation and in greenhouse for leaf blast resistance evaluation
The results showed that Jiangnanwan was highly resistance to panicle blast and leaf blast, while Suyunuo was highly susceptible (Fig 2A–2C, Table 1)
Fewer genetic analyses of rice panicle blast resistance have been reported compared with leaf blast resistance
Summary
We obtained 221 F2:7 RILs with three generations (F2:5, F2:6 and F2:7), and identified panicle blast and leaf blast resistance genes to the strain Hoku 1 with QTL mapping method. We examine the correlation between the resistance of panicle and leaf blast, and fine mapped the blast resistance gene Pi-jnw1.
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