Characterization and Evaluation of Antibacterial Activities of Chemically Synthesized Iron Oxide Nanoparticles

Sudhanshu Shekhar Behera,Hrudayanath Thatoi,Niladri Panda,Jayanta Kumar Patra,Krishna Pramanik

doi:10.4236/wjnse.2012.24026

Sudhanshu Shekhar Behera, Hrudayanath Thatoi + Show 3 more

Open Access

https://doi.org/10.4236/wjnse.2012.24026

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Abstract

The iron oxide nanoparticles have been synthesized in co-precipitation method using aqueous solution of ferric and ferrous ions with sodium salt. The synthesis of iron-oxide nanoparticles were validated by UV-Visible spectroscopy which showed higher peak at 370 nm as valid standard reference. An average size of iron oxide nanoparticle found by Diffraction Light scattering (DLS) particle size analyser, ranges approximately between 10 nm to 120 nm with mean particle size of 66 nm. The X-ray power diffraction (XRD) analysis revealed the crystallographic structure of magnetic particles. Characterization of the mean particle size and morphology of iron oxide nanoparticles confirmed that the iron oxide nanoparticles are nearly spherical and crystalline in shape. Further the antibacterial effect of iron oxide nanoparticles was evaluated against ten pathogenic bacteria which showed that the nanoparticles have moderate antibacterial activity against both Gram positive and Gram negative pathogenic bacterial strains and retains potential application in pharmaceutical and biomedical industries.

Highlights

Nanometer-size metallic nanoparticles have been the subject to research in recent years because these materials represent an intermediate dimension between bulk materials and atoms/molecules [1]
Further the antibacterial effect of iron oxide nanoparticles was evaluated against ten pathogenic bacteria which showed that the nanoparticles have moderate antibacterial activity against both Gram positive and Gram negative pathogenic bacterial strains and retains potential application in pharmaceutical and biomedical industries
The iron oxide nanoparticles (Fe3O4) synthesized by coprecipitation of ferric and ferrous chloride was validated by UV-Visible spectroscopic analysis and their scanning absorbance vs wave length (λ) has been established (Figure 2)

Summary

Introduction

Nanometer-size metallic nanoparticles have been the subject to research in recent years because these materials represent an intermediate dimension between bulk materials and atoms/molecules [1] Among these metallic nanoparticles, iron oxide (IO) have received special attention because of their variety of scientific and technological applications such as biosensor [2], antimicrobial activity [3], food preservation [4], magnetic storage media, ferrofluids, magnetic refrigeration, magnetic resonance imaging, hyperthermic cancer treatments, cell sorting and targeted drug delivery [5,6,7]. Nanoparticles have long been known to exhibit a strong toxicity to a wide range of micro-organisms [10, 12], very little is known about the toxicity of iron oxide nanoparticles towards these microorganisms

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