Characterization and Evaluation of a Denitrifying and Sulfide Removal Bacterial Strain Isolated From Daqing Oilfield

Abstract

Sulfate-reducing bacteria (SRB) and hydrogen sulfide were widely present in oil production systems, which are detrimental to oil exploitation industry. An anaerobic denitrifying and sulfide removal bacterial strain, identified as Pseudomonas stutzeri DQ1, was isolated from Daqing Oilfield. Starin DQ1 grew well at temperatures 20–40°C, pH 6.5–8.0, and salinity 0–50 g/L of NaCl. Strain DQ1 can completely remove sulfide less than 50.0 mg/L, and decrease the SRB numbers nearly four orders of magnitude. Bioaugmentation of strain DQ1 in oil production system will be a promising approach for simultaneous inhibition of SRB and removal of sulfide in Daqing Oilfield.