Characterization and enzymatic degradation of microbial copolyester P(3HB- co-3HV)s produced by metabolic reaction model-based system

Abstract

The two types of poly(3-hydroxybutyrate- co-3-hydroxyvalerate)s [P(3HB- co-3HV)s] were produced by Paracoccus denitrificans ATCC 17741 using two different feeding methods. The produced P(3HB- co-3HV)s were fractionated and the copolymer sequence distributions were analyzed by 1H and 13C NMR spectroscopy. It was found that the P(3HB- co-3HV) samples produced by conventional feeding method were statistically random copolymers. The sequence distributions of P(3HB- co-3HV) samples produced by optimization method were different from random P(3HB- co-3HV)s. The thermal properties and melting behaviors were analyzed by differential scanning calorimetry (DSC). These results demonstrated that P(3HB- co-3HV) samples produced by optimization method are close in nature to P(3HB- co-3HV)s rich in long-sequence of block 3HB units, but less in 3HV random regions. The enzymatic degradation profile of P(3HB- co-3HV) films was investigated in the presence of 3-hydroxybutyrate depolymerase from Pseudomonase lemoignei. The degradation process was observed by monitoring the time-dependent change in the weight loss of copolymer films. The surface erosion of copolymer films was qualitatively monitored by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The highest degradation rate of 2.6% per day was observed for random P(3HB- co-38%3HV) produced by conventional method. In comparison, the hydrolysis degradation rates of random P(3HB- co-3HV)s were about one time faster than those of P(3HB- co-3HV)s produced by optimization method.