Abstract
The most active and abundant phytotoxin formed by the plant parasitic fungus Bipolaris sorokiniana was prehelminthosporol (C 15H 22O 2)). The toxin is a hydrophobic sesquiterpene with restricted thermal stability and low water solubility (0.15 μg·μl −1). Prehelminthosporol was detected in conidia, hyphae and surrounding using “flash”-chromatography on silica gel and preparative HPLC. Purity controls were carried out on both straight-and reverese phase HPLC columns using RI detection. For quantification we used reverse phase on-line high performance liquid chromatography/mass spectrometry and plasma spray ionization. Using selected ion monitoring measuring at m/ z 219 a detection limit of ≈ 5 ng toxin was obtained.
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