Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina.

Fernando A Bessone,Javier Cappuccio,M B Conde,Marina Dibarbora,Gustavo Zielinski,Maria Laura Soriano Perez,Fabrisio Alustiza

doi:10.14202/vetworld.2019.434-439

Abstract

Background:Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry.Aim:This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains.Materials and Methods:Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles.Results:We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF.Conclusion:The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

Highlights

We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene
Progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs are the main diseases caused by Pasteurella multocida (Pm) [1]
Numerous critical genes for Pm virulence have been identified in an attempt to elucidate the pathogenesis of pasteurellosis, which remains as a puzzle in many of its host species such as pigs [3,4,5,6,7,8]

Summary

Introduction

Progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs are the main diseases caused by Pasteurella multocida (Pm) [1]. This agent is part of the porcine respiratory complex (PRC) and is responsible for significant economic losses at the productive level in porcine farms [2]. Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry

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Methods

Discussion

Conclusion