Characterization and chromosomal distribution of novel satellite DNA sequences of the lesser rhea (Pterocnemia pennata) and the greater rhea (Rhea americana).

Abstract

Two different types of novel satellite DNA (stDNA) sequences were cloned from the lesser rhea (Ptercnemia pennata) and the greater rhea (Rhea americana) after digestion of genomic DNAs with a restriction endonuclease Pvu II, and characterized by filter hybridization and in-situ hybridization to metaphase chromosomes. These nucleotide sequences consisted of GC-rich 288-bp and 332-bp repeated elements in P. pennata and 288-bp and 336-bp repeated elements in R. americana, all of which were organized in tandem arrays in the genome. The 288-bp and 332-bp elements of P. pennata displayed strong sequence similarity with the 288-bp and 336-bp elements of R. americana, respectively. The 332-bp and 336-bp elements were located on almost all the microchromosomes in both the species. The other type of repeated elements, the 288-bp element, was located on four and nine pairs of microchromosomes in P. pennata and R. americana, respectively. All the stDNA sequences were not crosshybridized to genomic DNAs of another three ratite species, ostrich (Struthio camelus), cassowary (Casuarius casuarius) and emu (Dromaius novaehollandiae), suggesting that these stDNA sequences are conserved in the same family but fairly divergent among the different families of Struthioniformes.