Characterization and Cellular Fluorescence Microscopy of Superparamagnetic Nanoparticles Functionalized with Third Generation Nanomolecular Dendrimers: In-vitro Cytotoxicity and Uptake study

Abstract

Characterization and Cellular Fluorescence Microscopy of Superparamagnetic Nanoparticles Functionalized with Third Generation Nanomolecular Dendrimers: In-vitro Cytotoxicity and Uptake study An optimal sample was selected as those synthesized at 70oC with particle size of about 10 nm << exchange length of 27 nm and a saturation magnetization of 67.8 emu/g. The samples were characterized with X-ray diffractometry (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, UV-vis spectroscopy, fluorescent spectroscopy (LIF) and magnetization measurements (VSM). The coated materials illustrated strong magnetic behaviour and XRD pattern like magnetite. The presence of Fe-O-Si bond in FTIR spectra confirmed the formation of thin APTS layer on the surface of magnetite nanoparticles. Thermogravimetric analysis (TGA) indicated that the modification of core synthesis technique can raise the efficiency of aminosilane coating reaction (as an initiator for PAMAM dendrimer) up to 98% with the production of about 610 dendritic arms. UV-vis spectrum of both SPIONs and ID-NPs was measured in the range of 340-380 nm with the maximum peak at about 350 nm. The fluorescence properties of ID-NPs distributed in a collagenous substrate and MCF 7 cells was studied by fluorescence microscopy. The results showed that the viability of L 929 and MCF 7 cells decreased from 100% and 90% to 53% and 23% respectively between 10 μg/mL and 1 mg/mL for ID-NPs. The rate of uptake increased with time and it was higher for ID-NPs than SPIONs.