Characterization and Cell Viability of Probiotic/Prebiotics Film Based on Duck Feet Gelatin: A Novel Poultry Gelatin as a Suitable Matrix for Probiotics.

Ahmadreza Abedinia,Najibeh Razgardani,Fazilah Ariffin,Farangis Teymori,Mohammad Reza Saeidi Asl,Abdorreza Mohammadi Nafchi,Faezeh Alimohammadi,Jumardi Roslan,Nurul Huda

doi:10.3390/foods10081761

Abstract

The probiotic viability, physicochemical, mechanical, barrier, and microstructure properties of synbiotic edible films (SEFs) based on duck feet gelatin (DFG) were evaluated. Four synbiotic systems were obtained by mixing four types of prebiotics, namely, dextrin, polydextrose, gum Arabic, and sago starch, with DFG to immobilize of probiotic (Lactobacillus casei ATCC). The ability of DFG to create a suitable matrix to increase probiotic viability was compared with those of other commercial gelatins in a preliminary evaluation. The DFG showed proper probiotic viability compared with other gelatins. The addition of prebiotics reduced the transparency of SEFs and increased color differentiation, uniformity, and complete coverage of probiotic cells. The estimated shelf-life of surviving bacteria in the SEFs stored at 4 and 25 °C showed that gum arabic showed the best performance and enhanced the viability of L. casei by 42% and 45%, respectively. Dextrin, polydextrose, and sago starch enhanced the viability of L. casei at 4 and 25 °C by 26% and 35%, 26% and 5%, and 20% and 5%, respectively. The prebiotics improved the physicochemical, mechanical, and barrier properties of all SEFs, except polydextrose film. The viability of L. casei can be increased with the proper selection of gelatin and prebiotics.

Highlights

IntroductionSome studies have reported limitations in terms of the probiotics dose
Origin on the Stability of L. casei. The of of this evaluation waswas to compare the ability of various gelatinsgelatins
Films made from duck feet gelatin (DFG) could replace gelatin derived from mammals in the generation of bioactive films

Summary

Introduction

Some studies have reported limitations in terms of the probiotics dose. The appropriate dose for the effective use of probiotics is less than (109 CFU/day). The validity of studies that do not consider the minimum consensus of appropriate probiotic dosages in their results is questioned [2]. Some of the probiotic benefits include the following: enhancement of the immune system, which in turn affects the severity and prevention of and susceptibility to COVID-19 [3]; protection against gastrointestinal pathogens [4]; reduction of serum cholesterol level and blood pressure [5]; anti-carcinogenic activity [6]; and the improvement of absorption and utilization of nutrients from food [7,8]

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Conclusion