Abstract
The polysaccharide obtained from Crataegus azarolus fruit, by water extraction followed by ethanol precipitation and fractionation using copper complex, shows physiological activities: prebiotic and antioxidant. The total acid hydrolysis shows that the polysaccharide consists of galactose, glucose, mannose and rhamnose. The employed physico-chemical techniques of the polysaccharide sequences approach (FT-IR, MALDI-TOF-MS spectrometry, 1H and 13C NMR) indicate that it is a Galactoglucomannan, the main chain consists of major amounts of β-(1→4)-linked glucose and mannose residues with monosaccharide branches of α-(1→6) galactose and O-acetyl substituents. The properties of this new glucomannan suggest that it could find an application as a prebiotic in the food industry.
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