Abstract
Pituitary-specific transcription factor (Pit-1) is responsible for pituitary development and hormone expression in mammals. In present study, a comparison of buffalo Pit-1 gene sequences with Bos taurus revealed no nucleotide changes however, 7 single nucleotide polymorphisms (SNPs), one of which was non-synonymous were found within Indian buffalo breeds (Bubalus bubalis). SNP c.160 T>A alters the encoded amino acid from methionine to lysine. Association of all SNPs with milk production traits was assessed in Mehsana buffalo populations and the results demonstrated that SNP c.160 T>A was significantly associated with the milk fat percentage (P=0.0358). Sequence analysis showed that the buffalo Pit-1 protein shares high homology with cattle (Bos taurus) (100%), goat (99%), sheep (99%), human (96%) and pig (98%). Polymorphisms detected in buffalo Pit-1 gene are expected to have functional significance and might be useful as genetic markers in association studies for milk fat and milk yield in buffalo.
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