Characterization and Antitumoral Activity of Biohybrids Based on Turmeric and Silver/Silver Chloride Nanoparticles.

Marcela-Elisabeta Barbinta-Patrascu,Andrada Lazea-Stoyanova,Nicoleta Badea,Gizo Bokuchava,Camelia Ungureanu,Alexander Zhigunov,Sanja Petrovic,Yulia Gorshkova,Yulia Gorshkova,Yulia Gorshkova,Mihaela Bacalum

doi:10.3390/ma14164726

Abstract

The phyto-development of nanomaterials is one of the main challenges for scientists today, as it offers unusual properties and multifunctionality. The originality of our paper lies in the study of new materials based on biomimicking lipid bilayers loaded with chlorophyll, chitosan, and turmeric-generated nano-silver/silver chloride particles. These materials showed a good free radical scavenging capacity between 76.25 and 93.26% (in vitro tested through chemiluminescence method) and a good antimicrobial activity against Enterococcus faecalis bacterium (IZ > 10 mm). The anticancer activity of our developed bio-based materials was investigated against two cancer cell lines (human colorectal adenocarcinoma cells HT-29, and human liver carcinoma cells HepG2) and compared to one healthy cell line (human fibroblast BJ cell line). Cell viability was evaluated for all prepared materials after a 24 h treatment and was used to select the biohybrid with the highest therapeutic index (TI); additionally, the hemolytic activity of the samples was also evaluated. Finally, we investigated the morphological changes induced by the developed materials against the cell lines studied. Biophysical studies on these materials were done by correlating UV–Vis and FTIR absorption spectroscopy, with XRD, SANS, and SAXS methods, and with information provided by microscopic techniques (AFM, SEM/EDS). In conclusion, these “green” developed hybrid systems are an important alternative in cancer treatment, and against health problems associated with drug-resistant infections.

Highlights

This article is an open access articleNano size particles formed from a reduced chemical element keep the properties of the used element as the main characteristics
UV–Vis absorption spectra displayed in Figure 1 revealed the spectral signature of silver nanoparticles (409 nm) and of chlorophyll (667 nm) in the biohybrids
The chitosan coating led to the appearance of more pronounced peaks (217; 251; 264 nm), due to the interaction between the amino and hydroxyl groups of chitosan and the functional groups of biocompounds derived from turmeric extract

Summary

Introduction

This article is an open access articleNano size particles formed from a reduced chemical element keep the properties of the used element as the main characteristics. The advanced properties of MNPs allow nanoparticles to be applied in various fields such as diagnostics, pharmaceutics, and therapy. Cadmium, titanium, and zinc oxide and cadmium sulphide nanoparticles [1]. Due to their good antimicrobial activity, silver nanoparticles (AgNPs) have been used in medicine, stomatology, textile, cosmetic, agriculture, and pharmaceutical industries [2,3]. Silver has been used as antibacterial agent against many microorganisms, but a large spectrum of new results about AgNPs’ use as antibacterial and antiviral agents [2,4,5,6,7] show that AgNPs have antibacterial but proven antiviral activity, as well to HIV-1 virus [8] and hepatitis b type [9]

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Results

Conclusion