Abstract
Objectives: The non-lactose fermenting Gram-negative bacilli (NLF-GNB) are notorious pathogens reportedly acquiring multiple drug resistance alarmingly and emerging as a public health threat globally. This study was conducted to isolate and identify these pathogens from clinical samples received routinely in our Bacteriology Laboratory and to analyze their antibiotic susceptibility patterns. Methods: In this cross-sectional study, the first 100 NLF-GNB strains isolated consecutively from 1218 clinical samples were included through convenience sampling. The samples were processed using standard microbiological techniques. Results: The most common isolate was Pseudomonas aeruginosa followed by Acinetobacter spp, Proteus spp, Shigella spp, Salmonella typhi, Providencia spp., and Morganella spp. P. aeruginosa and Acinetobacter spp. isolates were found to exhibit high susceptibility toward Colistin and Imipenem. Proteus spp. exhibited high sensitivity toward Imipenem, Aminoglycosides, Ceftazidime, and Cefepime. All Providencia isolates were susceptible to Amikacin, Cefepime, and Ceftriaxone. The only isolate of Morganella spp. was found to be susceptible to Amikacin, Cefepime, Ceftazidime, Piperacillin tazobactam, Ciprofloxacin, Imipenem, and Aztreonam. Shigella isolates exhibited very high susceptibility toward Imipenem followed by Gentamicin and Ceftazidime. All the isolates of S. typhi exhibited susceptibility toward Imipenem, Piperacillin tazobactam, Ceftazidime, Ceftriaxone, Cefoperazone sulbactam, and Chloramphenicol. 24% of test isolates were found to be Multidrug resistant. Conclusion: Antimicrobial surveillance is needed to implement appropriate timely interventions to restrict the spread of multidrug-resistant clones. Strict infection prevention and control practices, with judicious antibiotic prescription policy, may help in tackling this problem by obviating the selection pressure.
Highlights
Aerobic non-fermenting Gram-negative bacilli (NFGNB) are a taxonomically diverse group of bacteria that are either not capable of utilizing carbohydrates as an energy source or degrading them via oxidative pathway [1,2,3].NFGNB (Pseudomonas, Acinetobacter, Burkholderia, Stenotrophomonas, etc.) constitute about 15% of all clinical bacterial isolates
The majority of samples yielding non-lactose fermenting Gram-negative bacilli (NLF-GNB) test isolates belonged to the patients in the age group of 40–60 years (42%) followed by the patients in the age group of 20–39 years (34%)
Akbar et al 2014 in a similar study conducted in Peshawar, Pakistan, found that most of the test isolates were contributed by the females and patients in the 11–30 years age group [1,8,12,13,14]
Summary
Aerobic non-fermenting Gram-negative bacilli (NFGNB) are a taxonomically diverse group of bacteria that are either not capable of utilizing carbohydrates as an energy source or degrading them via oxidative pathway [1,2,3]. NFGNB (Pseudomonas, Acinetobacter, Burkholderia, Stenotrophomonas, etc.) constitute about 15% of all clinical bacterial isolates. NFGNBs are emerging as important health-care-associated pathogens in the current scenario. Hospital strains are found to exhibit multidrug resistance (MDR). They have been incriminated in infections, such as septicemia, meningitis, pneumonia, urinary tract infections, and surgical site infections. NFGNBs are innately resistant to many antibiotics and are known to produce extended-spectrum ß-lactamases (ESBL) and Metallo ß-lactamases [4,5,6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
