Characterization And Antimicrobial Property Of Nickel Nanoparticle Synthesized Using Leaves Extract Of Launaea Taraxacifolia (African Lettuce)

Abstract

As an alternative to previously used, costly, and hazardous chemicals, nickel nanoparticles (NiNPs) were manufactured utilizing locally accessible biodiversity plants. The aim of this work is to examine the antibacterial capabilities of nickel nanoparticles generated from Launaea taraxacifolia (African lettuce or wild lettuce). Leaves of African lettuce (Launaea taraxacifolia) were gathered in Esa-Oke, Nigeria's Osun State. In the morning, a sterile container was used to collect urine. The urine sample was evaluated and cultured using C.L.E.D. medium, MacConkey agar, and Sabouraud dextrose agar. Traditional methods including as morphology, gram staining, biochemical analysis, and sugar fermentation were used to identify the isolates. African lettuce was cut into small pieces after being washed in distilled water. A nickel nanoparticle was made using a conventional approach from African lettuce leaf extract. Transmission Electron Microscopy was used to characterize the nanoparticle, which was done via a drop coating method (SEM). The isolates were identified as Klebsiella sp. and Micrococcus sp. using Bergy's manual for bacterial identification. Nickel nanoparticles and African lettuce leaf extract were tested for antibacterial activities against Klebsiella sp. and Micrococcus sp. utilizing agar well diffusion methods. The comprehensive Transmission Electron Microscopy (TEM) photos clearly demonstrate that nickel nanoparticles have identical morphologies and sizes, according to the findings of the study of generated nanoparticles. Nickel nanoparticles with a diameter distribution of 10–20 nanometers are visible in the TEM images. The zones of inhibition for nickel nanoparticles against tested bacterial isolates (Klebsiella sp and Micrococcus sp) were 2 mm and 0 mm, respectively, however the zones of inhibition for Launaea taraxacifolia extract were substantial (5 mm and 7 mm). Plant extracts (Launaea taraxacifolia) showed good inhibition against bacteria when compared to the stated zone of inhibition with generated nickel nanoparticles (Micrococcus sp and Klebisiella sp)