Characterization and Antimicrobial Properties of Biofabricated Silver Nanoparticles Using Different Extracts of Boerhavia diffusa L.

Abstract

The benefit of utilizing plants for the synthesis of nanoparticles include a broad variability of metabolites that may aid in reduction, easily available as well as safe to handle. Boerhavia diffusa L. is known as punarnava, possessing anti-inflammatory, diuretic activities and is being used for the common clinical problems such as nephrotic edema, syndrome and ascites resulting from early cirrhosis of the liver and chronic peritonitis. The present study aims at biosynthesis of silver fabricated nanoparticles with the aqueous extracts of B. diffusa leaves and roots by using 1 mM AgNO3 . Preliminary confirmation of silver nanoparticles was done by using Ultra Violet Visual (UV-VIS) Spectrophotometer. The biomolecules were responsible for reducing the Ag+ to Ag0 which was identified by Fourier Transform Infra-Red (FTIR). The nanoparticles were subjected to Scanning Electron Microscopic (SEM) analysis to detect the Size and morphology of the particles. The nanoscale level (< 100 nm) of spherical shaped aggregates was identified. Antimicrobial assays were carried out with the selected clinical isolates using disc diffusion method and the notable inhibitory zones were observed against all the isolates at very low concentration.