Abstract
N. sativa is an interesting source of bioactive compounds commonly used for various therapeutic purposes. Associate its seeds extracts with biomaterials to improve their antimicrobial properties are highly demanded. This study aims to investigate the encapsulation of NS extracts in hydroxyapatite nanoparticle sodium silicate glass (nHap/SSG) scaffold. NS essential oil (HS) was extracted by hydrodistillation, while hexane (FH) and acetone extracts (FA) were obtained using Soxhlet extraction. (FH) was the most abundant (34%) followed by (FA) (2.02%) and (HS) (1.2%). GC-MS chromatography showed that the (HS) contained beta cymene, alpha thujene, β-pinene and thymoquinone, while (FH) had mostly fatty acids and (FA) decane, 2.9-dimethyl, benzene 1,3,3-trimethylnonyl and beta cymene. Loaded nHap/SGG scaffolds with various amount of (FH), (HS) and (FA) at 1.5, 3, and 6 wt%; were elaborated then characterized by ATR-FTIR, X-ray and SEM techniques and their antimicrobial activity was studied. Samples loaded with 1.5 wt% HE was highly active against C. albicans (19 mm), and at 3 wt% on M. luteus (20 mm) and S. aureus (20 mm). Additionally, loaded scaffolds with 1.5 wt% AE had an important activity against M. luteus (18.9 mm) and S. aureus (19 mm), while the EO had low activities on all bacterial strains. The outcome of this finding indicated that loaded scaffolds demonstrated an important antimicrobial effect that make them promising materials for a wide range of medical applications.
Highlights
Nigella sativa L., known as black cumin or black seed, is a plant that is bursting with interesting compounds responsible for medicinal proprieties [1]
3 wt%, the measured inhibition zone diameter against S. aureus was 20 ± 0.62 mm. These results suggest that even at low concentration (1.5 and 3 wt%) of added plant extracts an important inhibition zone is developed, which indicates an efficient antimicrobial activity
Extract (FA), which did not give any antimicrobial effect before its encapsulation [59], in this study showed its activity expand against C. albicans, M. luteus, and S. aureus
Summary
Nigella sativa L., known as black cumin or black seed, is a plant that is bursting with interesting compounds responsible for medicinal proprieties [1]. The (FH) and (FA) of N. sativa seeds were previously tested for their wide array of therapeutics activities such anti-tumor [4], antimicrobial [3,5], anti-inflammatory [6], antioxidant [7], anticancer [8]. Pretreatment with N. sativa seeds have shown a protective effect against kidney injuries [10]. N. sativa was reported to be useful for treating infected bones and enhances the healing process as well as preventing for mitigating infection risk [11,12].
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