Characterization and analysis of extracellular vesicle-derived miRNAs from different adipose tissues in mice

Abstract

Adipose tissue is traditionally classified into two main types based on their functions: brown adipose tissue (BAT) and white adipose tissue (WAT). Each type plays a distinct role in the body's energy metabolism. Additionally, a third type, beige adipose tissue, can develop within subcutaneous WAT (including inguinal WAT, iWAT) in response to specific stimuli and exhibits characteristics of both BAT and WAT. Extracellular vesicles (EVs) are crucial for intercellular communication, carrying a diverse array of biomolecules such as proteins, lipids, and nucleic acids. While the functional diversity and endocrine roles of adipose tissues are well-documented, a comparative analysis of the functions of EVs released by different adipose tissues from mice housed at room temperature has not been thoroughly explored. MicroRNAs (miRNAs), which are highly enriched in small extracellular vesicles (sEVs), offer a promising avenue for investigating the complex functions and unique roles of various adipose tissues. In this study, we isolated sEVs from different adipose tissues under basal conditions and performed a comprehensive analysis of their miRNA content. By comparing miRNA profiles across different adipose tissues, we aim to elucidate the potential roles of sEV-derived miRNAs in mediating intercellular communication and the distinct physiological functions of adipose tissues. Understanding the molecular features of miRNAs in adipose tissue EVs could reveal new aspects of adipose tissue biology and lay the groundwork for further research into their physiological significance.