Abstract
Butyrate-producing bacteria can biosynthesize butyrate and alleviate inflammatory diseases. However, few studies have reported that the genus Collinsella has the ability to produce butyric acid. Here, our study depicts a Collinsella strain, which is a rod-shaped obligate anaerobe that is able to produce butyric acid. This microorganism was isolated from a human gut, and the optimal growth conditions were found to be 37 °C on PYG medium with pH 6.5. The 16S rRNA gene sequence demonstrated that this microorganism shared 99.93% similarity with C. aerofaciens ATCC 25986T, which was higher than the threshold (98.65%) for differentiating two species. Digital DNA–DNA hybridization and average nucleotide identity values also supported that this microorganism belonged to the species C. aerofaciens. Distinct phenotypic characteristics between TF06-26 and the type strain of C. aerofaciens, such as the fermentation of D-lactose, D-fructose and D-maltose, positive growth under pH 5 and 0.2% (w/v) cholate, suggested this strain was a novel subspecies. Comparative genome analysis revealed that butyric acid kinase and phosphate butyryltransferase enzymes were coded exclusively by this strain, indicating a specific butyric acid-producing function of this C. aerofaciens subspecies within the genus Collinsella. Thus, Collinsella aerofaciens subsp. shenzhenensis subsp. nov. was proposed, with set strain TF06-26T (=CGMCC 1.5216T = DSM 105138T) as the type strain.
Highlights
The isolation and cultivation of microorganisms are rate-limiting steps in the study of microbiology
The objective of this study is to identify and characterize a new strain of a butyric acid-producing organism after isolating it from a human gut
Gas chromatograph showed that 4.37 mmol/L butyric acid, 19.88 mmol/L acetic acid, 38.67 mmol/L lactic acid and 2.19 mmol/L benzoic acid were produced after 72 h of fermentation in peptone–yeast extract–glucose (PYG) medium
Summary
The isolation and cultivation of microorganisms are rate-limiting steps in the study of microbiology. The study of intestinal bacteria isolation was boosted in the 1960s and 1970s by the emergence and use of anaerobic cultivation techniques [1]. The breakthroughs in modern sequencing technologies have enabled researchers to obtain more genomics information to identify new organisms. The number of isolated and cultivated gut microorganisms has increased considerably in recent years, especially butyric acid-producing bacteria. A product of intestinal microbial fermentation, plays an important role in colonic health and serves as an energy source for epithelial cells. Previous studies have suggested that butyric acid-producing bacteria could alleviate inflammatory bowel disease, type 2 diabetes and obesity [2,3,4]
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