Abstract
SUMMARY The ease of isolation of two mutants of Streptococcus faecalis 8043 appeared to be the result of the presence in the parent strain of a system for slow synthesis of lysine. The mutation to lysine independence occurred when the population became large enough for a significant number of spontaneous mutational events to occur. The slow growth and lysine depletion lysis of the parent strain selected for the new strain. The hydroxylysine-resistant mutant can arise from the parent strain or from the lysine-independent strain and is selected for by growth in a medium containing hydroxylysine. The three strains appeared to be identical by all tests employed except in their responses to lysine and hydroxylysine.
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