Abstract
Native liver ferritin of Dasyatis akajei (DALF), apoDALF, and reconstituted DALF were employed to construct a ferritin reactor, respectively. An apparatus consisting of a mixer, a ferritin reactor, and a magnetic stirrer was constructed to study capacity and feasibility of trapping Cu2+ in the flowing seawater. The experimental results showed that the numbers of trapping Cu2+ with DALF reactor were higher than these with the reactors of apoDALF and reconstituted DALF, respectively, giving the maximal numbers of 98 +/- 5 Cu2+ per molecular DALF in 120 h. We found that the iron layer with a high ratio of phosphate to ion on the surface of the ferritin core played an important role in increasing numbers of trapping Cu2+. In addition, we found two positive relations of dependence of trapping Cu2+ numbers with the reactor on the incubation time and on the Cu2+ concentration in the flowing seawater. Another apparatus consisting of a buoyage, an isolation basket equipped with griddling, and a scrolled ferritin reactor was constructed to study the feasibility of trapping Cu2+ in the sea area. Moreover, the present studies indicated that this apparatus had been used to not only analyze and evaluate the concentration variety of various heavy metal ions such as Cu2+ and Pb2+ diluting by the seawater but also monitor the formation of pollution degree by various small organic molecules during the climax and the neap.
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