Characteristics of the syrian hamster ribonucleic acid present in cells transformed by polyoma, simian virus 40, or adenovirus 12.

Abstract

Ribonucleic acids of known specific activities were separately prepared from hamster cells transformed by polyoma, simian virus 40 (SV40), and adenovirus 12. When increasing amounts of these labeled ribonucleic acids were added to nitrocellulose filters containing immobilized (14)C-hamster deoxyribonucleic acid (DNA), about 5% of the DNA sites were saturated in each case. An additive saturation-hybridization experiment indicated that a portion of the 5% of the hamster DNA transcribed in SV40-transformed cells was also transcribed in polyoma-transformed cells. In addition, a separate region of the hamster genome was also transcribed in polyoma-transformed cells.