CHARACTERISTICS OF THE RESULTS OF ELECTRICAL EXPOSURE WHEN INDUCING OSTEOMYELITIS IN RATS BY DIFFERENT TYPES OF PATIENTS

Abstract

The aim of the research. To study the features of the course of osteomyelitis in rats during the induction of an infectious and inflammatory process by various pathogens under conditions of early and delayed local exposure to direct electric current. Materials and methods. The study was carried out on 53 Wistar rats, divided into 3 series. In each series, osteomyelitis of the femur was modeled by direct inoculation into the femoral canal of a monobacterial culture (Staphylococcus aureus; MSSA, museum strain, or Pseudomonas aeruginosa, museum strain, or Escherichia coli, museum strain) at a concentration of 1 × 108 CFU and placing an intramedullary metal implant. The animals of each series were divided into 3 groups. In group 1, no additional manipulations were performed with the animals; in groups 2 and 3, the source of infection was exposed to a direct electric current (150 mAm, 1 minute) on days 0, 2, 4 and 6, or on days 7, 9, 11 and 13. The duration of the experiment was 21 days. Results. All animals had a clinical picture characteristic of osteomyelitis, but the nature, severity and frequency of occurrence of signs of the infectious-inflammatory process in the groups were different. Conclusion. Exposure to electric current early after infection had the most pronounced antimicrobial effect against P. aeruginosa when exposure began on day 7 after infection. A less pronounced effect of electrical exposure was noted in relation to S.aureus and E.coli when exposure began on the first day after infection.