Abstract
1. 1. Tubulin has been isolated from brain of carp ( Cyprinus carpio), acclimated to summer temperatures (16–20°C), and its in vitro reassembly behavior has been characterized. 2. 2. Among the striking properties of this tubulin preparation is the temperature profile showing a high level of polymerization at the environmental temperature of carp. 3. 3. The critical tubulin concentration for assembly was 0.8 mg/ml, which was higher than mammalian tubulin purified by the cycle procedure. 4. 4. The microtubular protein showed three high mol.wt component and a minor component of about 43,000 daltons was also found to copurify with tubulin.
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