Abstract
Aim. In order to determine the expression level of immunohistochemical markers of matrix metalloproteinase-9 (ММР-9) and its tissue inhibitor TIMP-1, and area of immunopositive cells а comprehensive histopathological, histological and immunohistochemical study of liver biopsy specimens was carried out in 94 patients, of which 55 (58.51%) patients had hepatocellular carcinoma and 39 (41.49%) – cholangiocellular carcinoma of liver.Methods and results. In 92.73% of patients with hepatocellular carcinoma and in 89.74% of patients with cholangiocellular carcinoma in malignant cells cytoplasmic expression of MMP-9 was determined. MMP-9 immunopositive cells took 59,33±22,57% of area of the tissue section of hepatocellular carcinoma and 52,71±20,86% of area of the tissue section of cholangiocellular liver cancer. In 80% of hepatocellular carcinoma patients and 74.36% of patients with cholangiocellular carcinoma in tumor cells cytoplasmic expression of TIMP-1 was revealed. TIMP-1 immunopositive cells constituted 21,94±6,27% of area of the tissue section of hepatocellular liver cancer and 33,05±13,85% of area of the tissue section of holangiocellular liver cancer.Conclusion. In both types of liver cancer direct strong relationship was noted between high levels of expression of MMP-9 by the tumor cells and low expression of TIMP-1, which indicates high invasive potential and aggressiveness of hepatocellular carcinoma and cholangiocellular carcinoma.
Highlights
З метою визначення рівня імуногістохімічної експресії матриксных металлопротеиназ (ММР)-9 та її тканинного інгібітора ТIМР-1, а також площі імунопозитивних клітин у холангіоцелюлярному й гепатоцелюлярному раку печінки здійснили патогістологічне, гістохімічне й імуногістохімічне дослідження трепанобіоптатів печінки 94 хворих, серед них 55 (58,51%) пацієнтів страждали на гепатоцелюлярний рак печінки, 39 (41,49%) – на холангіоцелюлярний рак печінки
In both types of liver cancer direct strong relationship was noted between high levels of expression of Matrix metalloproteinases (MMPs)-9 by the tumor cells and low expression of Tissue Inhibitors of Metalloproteinases (TIMPs)-1, which indicates high invasive potential and aggressiveness of hepatocellular carcinoma and cholangiocellular carcinoma
Высокий (134,04±21,47 условных единицах оптической плотности (УЕОП)) и умеренный (74,83±14,64 УЕОП) уровень экспрессии матриксной металлопротеиназы-9 (ММР-9) клетками холангиоцеллюлярной карциномы (ХЦК) установлен у одинакового процента больных, а низкий уровень экспрессии ММР-9 (32,88±9,60 УЕОП) отмечен у 28,21% пациентов
Summary
In 92.73% of patients with hepatocellular carcinoma and in 89.74% of patients with cholangiocellular carcinoma in malignant cells cytoplasmic expression of MMP-9 was determined. MMP-9 immunopositive cells took 59,33±22,57% of area of the tissue section of hepatocellular carcinoma and 52,71±20,86% of area of the tissue section of cholangiocellular liver cancer. In 80% of hepatocellular carcinoma patients and 74.36% of patients with cholangiocellular carcinoma in tumor cells cytoplasmic expression of TIMP-1 was revealed. TIMP-1 immunopositive cells constituted 21,94±6,27% of area of the tissue section of hepatocellular liver cancer and 33,05±13,85% of area of the tissue section of holangiocellular liver cancer
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