Characteristics of soluble microbial products and extracellular polymeric substances in the membrane bioreactor for water reuse

Abstract

In biological treatment processes, the effluent contains some portion of organic material originating from soluble microbial products (SMP) that is closely related with extracellular polymeric substances (EPS), although the biological activity is maximized. These SMP should be considered important target materials if additional processes are necessary to satisfy the required water quality for the intended reuse. In this study, the characteristic of SMP and EPS were investigated using a submerged membrane bioreactor, which is one of the most promising water reuse process. Most protein SMP in the reactor existed at a molecular weight (MW) above 10 kDa. Over 86% of the carbohydrate SMP contained in the permeate had a MW below 1 kDa. The protein and carbohydrate SMP in the permeate account for 83–91% of the COD. The MW of the protein and carbohydrate SMP in the reactor, when converted to COD showed a bimodal pattern (less than 1 kDa or greater than 10 kDa), but over 81% of those in the permeate existed below 1 kDa due to the membrane rejection. The relative hydrophobicity of the protein and carbohydrate SMP was affected by the influent characteristics, and the hydrophobicity was decreased after the membrane filtration. The total EPS concentrations increased with increases in the food to microorganism ratio in this study. The modified fouling index (MFI) was used to investigate the biofouling characteristics, in terms of soluble and suspended solid parts closely related with the SMP and EPS, respectively.