Characteristics of sawdust cultivation of Lentinula edodes with different methods of spawn inoculation

Abstract

This study was carried out to investigate the management characteristics and growth performance of L. edodes from the cooling stage to incubation. Bags of different heights and weights are available for bagging. When the medium size of 17x13 cm was used and the size of the inoculation hole was changed from 1/3 to 2/3, the browning period was shortened to 30 days. Mycelial growth was evaluated according to the cooling temperature after sterilization. It was observed to be the highest at 122 mm/15 days at 10oC and 114 mm/15 days and 117 mm/15 days at 15oC and 20oC, respectively. The contamination rate of the sawdust media before inoculation was measured as 0, 4.5x10, 1.3x102, 4.0x103 cfu at 5oC, 10oC, 15oC, and 24oC respectively. The average of 1.6x108 colony forming units (cfu) of microorganisms was observed in the sawdust that had been piled for six months outdoors. In summer, the sawdust has to be used immediately after mixing. The sterilized medium had an average of 4x103 cfu of microorganisms at 24oC and 1.3×102 cfu at 15 °C. After 15 days of inoculation in vitro, the growth conditions of the sawdust was the best at 132 mm, followed by grain and liquid. When inoculated with liquid spawn, the moisture content of the substrate should be adjusted between 50% and 55% in advance.