Characteristics of RNA synthesized in vitro by lymphocytes of chronic lymphocytic leukemia.

Abstract

Abstract Considerable amounts of RNA are synthesized in vitro by the lymphocytes of chronic lymphocytic leukemia. The material synthesized at 1 hour is quite stable, sediments faster than ribosomal RNA in, a sucrose gradient, and forms DNA-RNA hybrids to the extent of 30 per cent with complementary DNA. Time-course experiments demonstrated that there was a delay in conversion of rapidly sedimenting RNA into material with sedimentation velocities corresponding to ribosomal and soluble RNA when compared to similar species of RNA from KB cells, thoracic duct lymphocytes, and 3T3 mouse fibroblasts.