Characteristics of rna synthesis on mustine-damaged templates

Abstract

1 h in 1.5, 0.9, 0.6, 0.4, 0.3, 0.2, and 0.i M, then overnight against urea in Na-acetate buffer, followed by 4 h against i0 mM Tris-HCl buffer, pH 8.0. Phenylmethylsulfanyl fluoride was present in all solutions. Electrophoresis of the chromatin proteins was carried out by Laemmli's method [6]. The sedimentation calculation were based on Osterman's nomograms [2] and the number of RNA molecules was determined by the method of Cedar and Felsenfeld [5], The mean sedimentation constant was calculated by the equation