Characteristics of proline transport into R3230AC mammary tumor cells

Abstract

Cells separated by enzyme treatment of the R3230AC mammary carcinoma were used to characterize the entry of proline. These cells showed minimal changes in cell viability and intracellular volume and were found to be suitable for transport studies, since the v i of proline was maintained for at least 4 h when cells were stored at 37 or 4°C, or when transport was measured in the presence or absence of Na +. Proline was actively transported by these tumor cells, reaching a distribution ratio ([proline] intracellular/[proline] extracellular) of 20 after 2 h. Proline entry consisted of two processes, one saturable (carrier mediated) and the other, non-saturable. The carrier-mediated entry, K m = 0.83 mM and V = 151 · 10 −5 μmol/min per 5 · 10 6 was Na +-dependent, sensitive to pH and metabolic inhibitors, and completely inhibited by α-(methylamino)-isobutyric acid ( K i = 0.34 mM). Proline entry in the absence of Na + was 20% that in the presence of Na + and was found to be due to a non-saturable process, since (a) υ i of proline uptake in the absence of Na + increased linearly with increasing proline concentration and (b) was not suppressed by either 20 mM α-(methylamino)-isobutyric acid, 50 mM glycine + 20 mM phenylalanine, or 50 mM serine + 20 mM phenylalanine when proline uptake was measured in the presence or absence of Na +. Therefore, under the conditions studied, we conclude that proline transport appears to be restricted to the A (alanine-preferring) system. Furthermore, these cells should provide a suitable model to study the effect of hormonal manipulations on the amino acid transport process.