Characteristics of products for NK-cell infusion prepared by CD3 depletion followed by CD56 enrichment: requirements for rare event analysis

Abstract

functionality we re-incubated 3-days rested cells with antigens. Induction of IFN-g in up to 100% of T cells was observed demonstrating cells maintained their in vivo imprinted physiological role. We also monitored CD45RA, CD28, and CCR7 expression on pp65-peptide specific CD8+ T cells before and directly after the enrichment. The enrichment process did not induce phenotypic changes. Thus, the newly developed automated manufacturing process provides a product, where the original phenotypic and functional characteristics of virus-specific T cells are conserved. Hence this cellular product is expected to fight viral infections effectively upon adoptive transfer.