Abstract
An alkaline polygalacturonate lyase (PGL) from Bacillus subtilis 7-3-3, PelC, with diverse depolymerization abilities for different pectin substrates was found. The PGL activity of PelC decreased with increasing degree of methyl esterification of the substrate. PelA and PelC displayed notable synergistic effects in the enzymatic degumming of ramie fibers. Gum loss rates increased by 62% when PelC was used to replace up to three-eighths of the PelA dose (PelC, 60 U g−1 ramie fibers). To the best of our knowledge, this study is the first to report the synergistic action of members of polysaccharide lyase families 1 and 3, represented by PelA and PelC, respectively. The present paper provides new insights into the improvement and production of enzymes used in enzymatic degumming.
Highlights
Polygalacturonate lyases (PGLs, EC 4.2.2.2) catalyze the transeliminative cleavage of polygalacturonate, which generates D4:5 unsaturated oligogalacturonates [1]
Alkaline polygalacturonate lyase (PGL), mainly referring to PelC with a dose ratio of 5:3 (PelA), degrades pectins located in the primary cell wall and the middle lamella of higher plants; the enzyme plays an important role in enzymatic degumming [3,4]
Phylogenetic Analysis of PelC PelC in B. subtilis 7-3-3 was classified as a member of polysaccharide lyase (PL) family 3 according to the results of BlastP analysis
Summary
Polygalacturonate lyases (PGLs, EC 4.2.2.2) catalyze the transeliminative cleavage of polygalacturonate, which generates D4:5 unsaturated oligogalacturonates [1]. Alkaline PGL, mainly referring to PelA, degrades pectins located in the primary cell wall and the middle lamella of higher plants; the enzyme plays an important role in enzymatic degumming [3,4]. A potential alternative to chemical degumming, presents several advantages, including flexible operation, limited damage to fibers, easy quality control [5], low energy consumption, and low environmental pollution. Enzymatic degumming in the textile industry has developed slowly because of the high cost and partial degumming performance of the available enzymes. Most microbial or enzymatic degumming processes are combined with a chemical process to achieve better degumming efficiency and meet the requirements of the textile industry [6,7]. Development of an enzyme system that can enhance the efficiency of the degumming process can promote the industrial-scale applications of enzymatic degumming
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