Abstract
Strain Y74T was an isolate from the sandy soil in the town of Huatugou, Qinghai–Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genus Planococcus. Strain Y74T was able to grow between 4 and 42°C (with an optimum temperature of 28°C) at pH values of 6–8.5 and in 0%–7% (w/v) NaCl. The dominant quinones were MK‐8 and MK‐7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The majority of the fatty acid content was anteiso‐C15:0 (28.8%) followed by C16:1 ω7c alcohol (20.9%) and iso‐C14:0 (13.4%). The 16S rRNA gene sequence similarity analysis demonstrated a stable branch formed by strain Y74T and Planococcus halotolerans SCU63T (99.66%). The digital DNA–DNA hybridization between these two strains was 57.2%. The G + C content in the DNA of Y74T was 44.5 mol%. In addition, the morphological, physiological, and chemotaxonomic pattern clearly differentiated the isolates from their known relatives. In conclusion, the strain Y74T (=JCM 32826T = CICC24461T) represents a novel member of the genus Planococcus, for which the name Planococcus antioxidans sp. nov. is proposed. Strain Y74T was found to have potent antioxidant activity via its hydrogen peroxide tolerance and its 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical‐scavenging activity. The DPPH radical‐scavenging activity was determined to be 40.2 ± 0.7%. The genomic analysis indicated that six peroxidases genes, one superoxide dismutase gene, and one dprA (DNA‐protecting protein) are present in the genome of Y74T.
Highlights
The accumulation of free radicals in living organisms can lead to many diseases, such as cancer and neurodegenerative diseases (Fischer & Maier, 2015; Lin & Beal, 2006)
In order to prevent the toxic effects of free radicals, potent natural antioxidants have been an important target for researchers
This environment is a potential source of genetic diversity and is an ideal place to search for antioxidant-producing microbes (Zhang, Wu, et al, 2016)
Summary
The accumulation of free radicals in living organisms can lead to many diseases, such as cancer and neurodegenerative diseases (Fischer & Maier, 2015; Lin & Beal, 2006). There were 16 valid species in the genus Planococcus until recently: P. citreus (Migula, 1895), P. kocurii (Hao & Komagata, 1985), P. antarcticus (Reddy et al, 2002), P. maritimus (Yoon, Weiss, Kang, Oh, & Park, 2003), P. maitriensis (Alam, Singh, Dube, Reddy, & Shivaji, 2003), P. rifietoensis (Romano, Giordano, Lama, Nicolaus, & Gambacorta, 2003), P. columbae (Suresh, Mayilraj, Bhattacharya, & Chakrabarti, 2007), P. donghaensis (Choi et al, 2007), P. salinarum (Yoon, Kang, Lee, Oh, & Oh, 2010), P. halocryophilus (Mykytczuk, Wilhelm, & Whyte, 2012), P. plakortidis (Kaur et al, 2012), P. soli (Luo et al, 2014), P. faecalis (Kim, Kang, Yu, Kim, & Lee, 2015), P. ruber (Wang et al, 2017), P. salinus (Gan, Zhang, Tian, et al, 2018), and P. halotolerans (Gan, Zhang, Zhang, et al, 2018). Strain Y74T demonstrated a strong antioxidant activity, which has potential antioxidant applications
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