Characteristics of molecular biology and gene mutations detections in core binding factor acute myeloid leukemia

Abstract

Objective To evaluate the value of the characteristics of detections in core binding factor acute myeloid leukemia (CBF-AML) with RUNX1-RUNXT1 or CBFB-MYH11 rearrangement.Methods Morphological,flow cytometric immunophenotyping,G-binding technique and FLT3-ITD mutation gene were performed in one hundred AML with RUNX1-RUNXT1 and thirty AML with CBFB-MYH11.Results The values of FLT3-ITD mutation in the AML with RUNX1-RUNXT1 and CBFB-MYH11 rearrangement were not significantly different [8 ％ (8/98),7 ％ (2/30),respectively].Other detections,in the CBF-AML with RUNX1-RUNXT1,CD34 93 ％ (91/98),CD117 89 ％ (88/98),CD19 39 ％ (38/98),CD15 11％ (11/98),additional chromosomal abnormalities 41％ (40/98),in the CBF-AML with CBFB-MYH11,CD34 33 ％ (10/30),CD117 23 ％ (7/30),CD11b67 ％ (20/30),CD1427 ％ (8/30),CD6427 ％ (8/30),additional chromosomal abnormalities (6 ％).CD7 were low in both [8 ％ (8/98),7 ％ (2/30),respectively],but in CBF-AML with FLT3-ITD mutation was high [40 ％ (4/10)].Conclusions Through the above comparison,two CBF-AML detection results are significantly different,suggesting that the mechanisms underlying the cause of leukemia is a different developmental trajectory.FLT3-ITD and CD7 are low in the two CBF-AML,that are associated the favorable prognosis of CBF-AML,and provides some reference for the study of individual treatment. Key words: Core binding factors; Leukemia, myeloid, acute; Immunophenotyping; CD7; FLT3-ITD