Characteristics of &lt;i&gt;TaFTSH6&lt;/i&gt; Gene and Expression Analysis under Dry and Heat Stress in Wheat

Yuan Li,Yunguo Liu,Huijun Jiang,Jun Liu,Xiaojun Hu

doi:10.5376/tgg.2021.12.0001

Abstract

In order to study the characteristics and functions of wheat FTSH genes, TaFTSH6 was cloned from wheat "bobwhite". Phylogenetic tree analysis showed that the amino acid sequence was very similar to FTSH6 sequence of other species. SMART analysis showed that TaFTSH6 had a conserved N-terminal transmembrane domain, ATP binding sites and hydrophilic Zn 2+ metalloproteinase domain. The gene was located on chr7D chromosome of wheat, named as TaFTSH6-7D . Cis -elements analysis of TaFTSH6 promoter sequence showed that there are several drought response sites CAACTG and a heat stress response site GACnnCTCnnGAA in the promoter, as well as many cis -elements related to hormone signal transduction. Quantitative expression analysis of TaFTSH6 gene in wheat seedlings under drought and heat stress showed that both drought and heat stress could increase the expression level of TaFTSH6 gene in root and leaf tissues, which indicated that TaFTSH6 is induced by drought and heat stress. Further study on the function of TaFTSH6 gene in wheat will partly support theoretical guidance to wheat breeding for drought and heat stress tolerance.

Highlights

IntroductionFTSH (filamentation temperature-sensitive H) is a cytoplasmic membrane protein endopeptidase and belongs to the AAA (ATPase associated with diverse cellular activities) protease family (Ogura and Wilkinson, 2001)
FTSH is a cytoplasmic membrane protein endopeptidase and belongs to the AAA (ATPase associated with diverse cellular activities) protease family (Ogura and Wilkinson, 2001)
Quantitative expression analysis of TaFTSH6 gene in wheat seedlings under drought and heat stress showed that both drought and heat stress could increase the expression level of TaFTSH6 gene in root and leaf tissues, which indicated that TaFTSH6 was induced by drought and heat stress

Summary

Introduction

FTSH (filamentation temperature-sensitive H) is a cytoplasmic membrane protein endopeptidase and belongs to the AAA (ATPase associated with diverse cellular activities) protease family (Ogura and Wilkinson, 2001). FTSH gene was first independently discovered by 4 different research groups when screening different phenotypes in Escherichia coli (Schumann, 1999) It has been found and studied in a variety of organisms such as Bacillus subtilis (Deuerling et al, 1995), Lactococcus lactis (Duwat et al, 1995), Arabidopsis thaliana (Lindahl et al, 1996), Nicotiana tabacum (Seo et al, 2000), Medicago sativa (Ivashuta et al, 2002), Citrullus lanatus (Akashi et al, 2004), Synechocystis sp (Kamata et al, 2005), Solanum lycopersicum (Sun et al, 2006a), Zea mays (Andjelkovic and Thompson 2006), Spinacia oleracea (Yoshioka et al, 2006), Brassica juncea (Knight et al, 2006), Solanum tuberosum (Fan et al, 2007), Xerophyta viscose (Ingle et al, 2007), Oryza sativa (Zhang and Sun, 2009), Glycine max (Yin et al, 2011), Lactobacillus plantarum (Bove et al, 2012), Arachis hypogaea (Zheng et al, 2016, Jiangsu Agricultural Sciences, 44(12): 74-77) and Acidovorax citrulli (Ji et al, 2019). In order to study the characteristics and functions of wheat FTSH genes, TaFTSH6 was cloned from wheat ‘Bobwhite’, and its expression changes under drought and heat stress were analyzed by bioinformatics to provide a theoretical basis for wheat drought and heat resistance and provide a new idea for wheat breeding for drought and heat stress tolerance

Methods

Results

Conclusion