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Abstract
The new lipoprotein analysis method using a cation-exchange chromatography, which contains a sulfopropyl-ligand column and two magnesium ion-containing eluents was previously reported. This method can separate serum lipoproteins on the column gel with a magnesium ion concentration gradient and high-density lipoprotein (HDL), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and an unspecified lipoprotein peak are eluted in order from the column. We have now characterized the unspecified lipoproteins, designated peak x, which is last eluted from the column with the eluent of high-magnesium ion concentration. The peak x was small size chylomicron fraction with a part of VLDL. Furthermore, the cholesterol values in the peak x were significantly correlated with remnant-like particle (RLP)-cholesterol values. The peak x separated from a hyperlipidemic patient included apolipoprotein B-100, B-48, E, A-1 and Cs (C-I, C-II, C-III) and its composition of free cholesterol, cholesteryl esters, triglyceride (TG) and phospholipids in total lipids were 6, 15, 66 and 13%, respectively. These results suggest that a major part of the lipoprotein peak X may be composed of remnants of chylomicron and VLDL, but it remains to be elucidated.
Highlights
Hyperlipidemia is a risk factor for atherosclerotic events, i.e., coronary heart disease, cerebral infarction and pulmonary infarction[1,2,3,4,5]
Likely to be hardly eluted from the column. These results suggest that the density of lipoproteins in peak x is
The peak x lipoproteins were appeared in VLDL and chylomicrons (Fig. 2A d, e and 2B d, e) of which retention times were 20.86, 20.98, 21.07 and 21.25, respectively
Summary
Hyperlipidemia is a risk factor for atherosclerotic events, i.e., coronary heart disease, cerebral infarction and pulmonary infarction[1,2,3,4,5] These patients often exhibit abnormal metabolism of lipids, including cholesterol, triglyceride (TG), phospholipids and cholesteryl esters[6], so that measurement of lipoprotein classes, high density lipoprotein (HDL), low density lipoprotein (LDL), intermediate-density lipoprotein (IDL), very low density lipoprotein (VLDL), chylomicrons is clinically important. In sera of Coronary Heart Disease (CHD) patients, remnants of chylomicrons and VLDL, including IDL, are increased[18,19,20,21] These TG rich lipoproteins are cataborized products from chylomicrons and VLDL under the interaction with lipoprotein lipase[19, 20]. The characteristics of the lipoprotein peak x were not well known and thereby some details about the peak x were investigated in the present study
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