Abstract
BackgroundSilages, as a nutritious and long-term preserved fodder, is achieved through fermentation. However, the constant low temperatures and freeze–thaw event are key factors that affect the quality of silages due to low levels of lactic acid production and undesirable microbial community revitalization. In this study, we attempt to investigate the effects of Lactobacillus plantarum QZW5 on the fermentation characteristics of wheat silage under ensiling conditions at the order of the following temperatures: constant low temperatures (5 °C for 30 days), multigelation (10 ℃ at day and – 10 ℃ at night for 30 days) and room temperature (aerobic/anaerobic, 10 days).ResultsUnder multigelation condition, Lactobacillus plantarum QZW5 inoculation significantly increased the contents of lactic acid and acetic (P < 0.05) in the silage. The pH, bacterial richness index (Chao) and diversity index (Shannon) of the silage were significantly decreased by Lactobacillus plantarum QZW5 (P < 0.05). In Lactobacillus plantarum QZW5 inoculated silage, the dominant genera were L. plantarum subsp. plantarum. However, those in the control silage were Leuconostoc mesenteroides and Leuconostoc fallax. LAB (lactic acid bacteria) inoculation increased the abundance of desirable Lactobacillus spp. and inhibited the growth of undesirable Escherichia coli, Saccharomyces cerevisiae, and Filamentous fungi, among others in the silage.ConclusionsTherefore, inoculation of L. plantarum QZW5 during ensiling could stimulate favorable fermentation and reconstruct bacterial community for better silage preservation.Graphic abstract
Highlights
Ensiling, as a popular method for plant material storage under acidic conditions, can ferment plant sugars to lactic acids using lactic acid bacteria (LAB) to achieve long-term nutrients preservation of unfermented feed
Characteristics of strain QZW5 It has been reported that the organic acid produced by LAB exhibits resistant effects on certain pathogens
Previous studies have shown that the moisture, crude protein, fat, Neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents in silage do not significantly change overall [2]
Summary
As a popular method for plant material storage under acidic conditions, can ferment plant sugars to lactic acids using lactic acid bacteria (LAB) to achieve long-term nutrients preservation of unfermented feed. (2021) 8:52 who has characteristics, including tolerance to stress conditions (e.g., acidic/alkaline conditions, high/low temperature, etc.) and capability of producing compounds against undesirable microorganisms, could be employed as microbial additives to prevent spoilage of silages [8]. LAB-related additives have been proved that they can significantly improve the fermentation quality of silages and inhibited its nutrition losing [9]. Find a suitable LAB-related additive combination for recurrent freezing and thawing conditions has crucial role in silages quality. The constant low temperatures and freeze–thaw event are key factors that affect the quality of silages due to low levels of lactic acid production and undesirable microbial community revitalization. We attempt to investigate the effects of Lactobacillus plantarum QZW5 on the fermentation characteristics of wheat silage under ensiling conditions at the order of the following temperatures: constant low temperatures (5 °C for 30 days), multigelation (10 °C at day and – 10 °C at night for 30 days) and room temperature (aerobic/anaerobic, 10 days)
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