Characteristics of induced spreading depression after transient focal ischemia in the rat

Abstract

We examined characteristics of spreading depression (SD) induced on the rat cortex 1 day after transient focal ischemia. Male Wistar rats ( n=21) were subjected to transient intraluminal thread occlusion of the right middle cerebral artery for 75 min. Twenty-four hours after the reperfusion, cerebral blood flow (CBF) was determined using laser Doppler flowmeter during multiple SDs elicited on both non-stroke (left) and stroke (right) cortex by the topical application of 2 M KCl. We also examined CBF responses before and after the intravenous administration of the nonspecific NOS inhibitor Nω-nitro- l-arginine methyl ester ( l-NAME, 10 mg/kg) in normal and stroke cortex. Animals were divided into two groups; Group 1 ( n=12), animals with subcortical infarction and Group 2 ( n=9), animals with subcortical plus cortical infarction. There were no differences between non-stroke and stroke sides in the duration or amplitude of the DC potential shifts in either group. The transient CBF hyperemia during SD was not different between non-stroke (372±23% of baseline, mean±S.E.) and stroke sides (383±30%) in Group 1. However, in Group 2, CBF was significantly restricted on the stroke side (192±15% vs. non-stroke side, 374±33%). In four normal animals without ischemia, there were no differences in CBF response between both sides. l-NAME had no effect on the transient CBF hyperemia during SD in any of the groups. These data suggest that the CBF responses during SD in the peri-infarction area is restricted 1 day after the transient focal ischemia, while CBF responses are intact in normal cortex overlapping a subcortical infarct. Further, our results indicate that nitric oxide does not promote CBF responses during SD in normal cortex or in tissue surrounding infarction.