Characteristics of glucose oxidase immobilized on Magnetic Chitosan Nanoparticles

Mehrdad AZIN,Behrouz AKBARI-ADERGANI,Rezvan POURAHMAD,Maryam EIN ALI AFJEH

doi:10.1590/fst.32618

Abstract

Abstract Immobilization process is used to facilitate the enzyme recovery and reusability. The aims of this study were to evaluate the effect of different amounts of chitosan on immobilization efficiency of Glucose Oxidase (GOX), pH effect on enzyme activity and changes of kinetic characteristics of enzyme immobilized on Magnetic Chitosan Nanoparticles (MCNP). GOX was immobilized on MCNP with cross bonds and Schiff base covalent connections. The sample with 0.3 g of chitosan had the highest immobilization efficiency (41.30%). Morphology of nanoparticles was investigated by Scanning Electron Microscopy (SEM). Immobilization of GOX on magnetic nanoparticles was verified with Fourier Transform Infrared (FTIR). The effect of pH on enzyme activity was similar on both immobilized enzyme (IE) and free enzyme (FE). The evaluation of kinetic parameters for IE and FE has showed that the effectiveness factor was 0.45. Generally, it could be concluded that magnetic nanoparticles with 0.3 g chitosan could provide a suitable medium for immobilization of GOX.

Highlights

Enzymes are biodegradable components which have less toxic effect in comparison to other prevalent industrial catalysers
Magnetic nanoparticles are considered more among all nanoparticles due to convenient separation with an outer magnetic field and their higher capacity to utilize for different purposes such as producing an advanced component, medical purposes, diagnostic methods, energy and food industries (Van Amerongen et al, 2005; Franzreb et al, 2006; Jeong et al, 2007)
Some of the advantages of this replacement are: a) Simple, fast and low-cost separation of enzymes from complex medium while inducing magnetic field (Liu et al, 2011); b) High enzyme loading capacity due to large special surface area; c) No limitation in penetration into the solvents (Kim et al, 2006). some researchers investigated stability and activity improvement of Glucose Oxidase (GOX) immobilized on modified iron oxide

Summary

Introduction

Enzymes are biodegradable components which have less toxic effect in comparison to other prevalent industrial catalysers. A lot of investigations have been conducted to immobilize GOX in different matrixes. Magnetic nanoparticles refer to particles with independent nature and magnetic element by size up to 100 nm These components have specific physicochemical properties which are dramatically different from bulk of the material (Osaka et al, 2006). Magnetic nanoparticles are considered more among all nanoparticles due to convenient separation with an outer magnetic field and their higher capacity to utilize for different purposes such as producing an advanced component, medical purposes, diagnostic methods, energy and food industries (Van Amerongen et al, 2005; Franzreb et al, 2006; Jeong et al, 2007). Some of the advantages of this replacement are: a) Simple, fast and low-cost separation of enzymes from complex medium while inducing magnetic field (Liu et al, 2011); b) High enzyme loading capacity due to large special surface area; c) No limitation in penetration into the solvents (Kim et al, 2006). some researchers investigated stability and activity improvement of GOX immobilized on modified iron oxide

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Conclusion