Abstract
A high titer fluorescein labelled antiglobulin preparation was compared with an antiglobulin of lesser potency in fluorescent treponemal antibody (FTA) titrations. The former gave higher FTA titers than the latter and therefore detected fewer antibody molecules per treponemal antigen particle. In addition, determination of the working titer of a labelled globulin by the procedure recommended by the USPHS was found to be influenced by the treponemal antibody content of the reference serum used for the titration. Moreover, titration of a labelled antiglobulin with a reference serum of high antibody content yielded a working titer that was too dilute for optimal antibody detection. FTA-ABS test results varied when antiglobulins were used at different working titers obtained in this fashion.
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