Abstract
Epstein-Barr virus (EBV) will infect at least every third cell if exposed in vitro to an extensively purified B cell population from human peripheral blood. About 10% of such infected cells will be driven into immunoglobulin synthesis and secretion, as judged by the indirect protein A plaque assay. The appearance of EB nuclear antigen, de novo DNA synthesis, and immunoglobulin secretion are linked phenomena accompanying infection as judged by viral dilution experiments, which yield kinetics of a one-hit order. Induction of immunoglobulin secretion in B cells by EBV requires de novo synthesis of DNA, and consequently, nontransforming EBV (P3HR1) will not induce immunoglobulin secretion and will also specifically block such induction from subsequently added EBV. The termination of immunoglobulin induction by EBV in short-term cultures appears to be T cell dependent.
Highlights
We demonstrate that there is a definite correlation between the induction of viral antigens, de novo synthesis of cellular DNA, and Ig secretion of in vitro Epstein-Barr virus (EBV)-infected B cells
Relationship between Ig Production, De Novo DNA Synthesis, and Intracellular Presence of Viral Antigens in B Lymphocytes Exposed to EBV
We have determined by these experiments that the EBV-induced activation of humanB lymphocytes is a dose-dependent, one-hit phenomenon, and that the maturational step involved in the process of activation requires de novo DNA synthesis
Summary
Mononuclear leukocytes were obtained from the blood of adult healthy donors. They were separated from the buffy coat of citrate blood on Ficoll-Isopaque gradients [6]. T and B cells were fractionated by erythrocyte rosette separation. Lymphocytes binding sheep erythrocytes (SRBC) were spun aAbbreviatzons used in this paper: Ara-C, cytosine arabinoside; EBNA, Epstein-Barr nuclear antigen; EBV, Epstein-Barr virus; FBS, fetal bovine serum; PFC, plaque-forming cells; PWM, pokeweed mitogen; SRBC, sheep erythrocytes
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