Abstract
Microbially induced carbonate precipitation (MICP) has a wide range of applications, and research on the mechanism of bacterial action has a positive effect on the efficient application of this technology. In this study, four different mineralized bacteria were selected, and the bacterial solution cultured for 30 h, the OD value of the bacterial solution reaches the peak. The results of fluorescence dip staining and flow cytometry show that the bacteria in the bacterial solution (30 hours) were mainly living cells, and the SEM images show that the cell sizes of the four bacteria are significantly different. This study also tested the reproductive characteristics of bacteria under different calcium ion concentrations. The results show that when the calcium ion concentration is 60 mmol/L, the bacteria can grow well, so biomineral formation is induced at this concentration. In order to analyze the effect of different components in the bacterial solution on calcium carbonate, the metabolites and cells are separated. The results of ultraviolet spectrum, infrared spectrum and hydrogen nuclear magnetic resonance spectrum show that the metabolites are mainly proteins. The results of SEM and CLSM images show that both cells and metabolites are involved in the formation of biominerals, and the bio-calcium carbonate induced by Bacillus pasteurii is smallest, its size is less than 2 μm.
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