Abstract
The goal was to gain understanding of how 12 genes containing SNP previously related to embryo competence to become a blastocyst (BRINP3, C1QB, HSPA1L, IRF9, MON1B, PARM1, PCCB, PMM2, SLC18A2, TBC1D24, TTLL3 and WBP1) participate in embryonic development. Gene expression was evaluated in matured oocytes and embryos. BRINP3 and C1QB were not detected at any stage. For most other genes, transcript abundance declined as the embryo developed to the blastocyst stage. Exceptions were for PARM1 and WBP1, where steady-state mRNA increased at the 9–16 cell stage. The SNP in WBP1 caused large differences in the predicted three-dimensional structure of the protein while the SNP in PARM1 caused smaller changes. The mutation in WBP1 causes an amino acid substitution located close to a P-P-X-Y motif involved in protein-protein interactions. Moreover, the observation that the reference allele varies between mammalian species indicates that the locus has not been conserved during mammalian evolution. Knockdown of mRNA for WBP1 decreased the percent of putative zygotes becoming blastocysts and reduced the number of trophectoderm cells and immunoreactive CDX2 in the resulting blastocysts. WBP1 is an important gene for embryonic development in the cow. Further research to identify how the SNP in WBP1 affects processes leading to differentiation of the embryo into TE and ICM lineages is warranted.
Highlights
During the preimplantation period, the mammalian embryo undergoes a series of morphological, molecular, physiological and metabolic processes that transform a single-cell totipotent zygote into a multicellular blastocyst composed of pluripotent inner cell mass and differentiated trophectoderm [1]
The surface of each ovary was sliced with a scalpel to harvest immature cumulus-oocyte complexes (COC) into oocyte collection medium
Transcripts were detected for 10 of the 12 genes evaluated; BRINP3 and C1QB were not detected at any stage
Summary
The mammalian embryo undergoes a series of morphological, molecular, physiological and metabolic processes that transform a single-cell totipotent zygote into a multicellular blastocyst composed of pluripotent inner cell mass and differentiated trophectoderm [1]. Embryonic development genes and analysis, decision to publish, or preparation of the manuscript
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