Abstract
The surface structure of an artificial membrane composed of a Millipore filter and a synthetic lipid analogue, dioleylphosphate (DOPH), immersed in KCl solution, changes when the concentration of CaCl 2 added to the KCl solution on one side of the membrane reaches a critical value. Using a photomultiplier mounted on the eyepiece of a stereoscopic microscope the structure change can be measured quantitatively as a variation of the intensity of the light reflected from the membrane surface. In the presence of calcium ions, the intensity decreased to approximately 50% of the value in the original hydrophilic state of the membrane in KCl solution only. However, the degree of structural change was dependent on adsorption density of DOPH, solution temperature and solution pH. On the other hand, using the light reflectance curves obtained from the structural observations, the concentration of an unknown amount of CaCl 2 in a KCl solution can be determined. Since the reflectance curves shift significantly with variation of the density of DOPH adsorbed on the Millipore filter, it is possible to detect a wide range of CaCl 2 concentrations, the detection range stretching from 0.1 to 50 m M CaCl 2.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
