Characteristics of blood proteome changes in hemorrhagic syndrome after head-up tilt test during 21-day Dry Immersion

Abstract

The effect of the rapid redistribution of blood during head-up tilt tests in 21-day Dry Immersion suddenly became relevant in connection with the occurrence of mild hemorrhagic syndrome in the study participants. It became necessary to analyze the processes of occurrence of hemorrhagic syndrome as orthostatic and congestive purpura. The aim of this work was to study the features of the proteomic response of the human body to the rapid redistribution of blood during head-up tilt tests during 21-day immersion, which led to stagnant and orthostatic purpura. According to the results of examination of 10 healthy male volunteers aged 23–34 years after head-up tilt tests, pitting edema and local petechial hemorrhages in the soft tissues of the legs and feet were observed. Proteomic analysis of the samples was carried out by high performance liquid chromatography with tandem mass spectrometry. For bioinformatic analysis, the software packages Perseus, ANDSystem were used. From reliably different (p-value <0.05) proteins, proteins directly related to injury and changes in vascular permeability were determined. Сhanges in the levels of plasminogen, fibronectin, other factors of coagulation and fibrinolysis, activation of the complement system, changes in the coagulation cascade and concentrations of plasminogen and its activators (kallikrein, etc.), increased content of fibrinolysis products were revealed. A panel of proteins indicating changes in plasmin-antiplasmin, fibrinogen-soluble fibrin-monomeric complexes and other systems is identified. The obtained data are of scientific and practical interest in the direction of stratification of the risk of secondary hemosiderosis and evaluation of new treatment methods aimed at reducing reperfusion damage to soft tissues and parenchymal organs.