Abstract
Phototherapy using light-emitting diodes (LEDs) centered on the green spectrum, which has a high cyclobilirubin production rate, was as effective as that centered on the blue spectrum for neonatal hyperbilirubinemia. There are no reports of species differences in bilirubin photochemical changes in this spectrum, and the characteristics of bilirubin photochemical changes in humans must be elucidated to proceed with the development of new light sources that include these spectra. This report describes the characteristic photochemical kinetics of bilirubin under green-spectrum LEDs in human, rat, rabbit, dog, pig, sheep, bovine and chicken serum albumin and rhesus monkey serum. These albumin-bilirubin complex solutions were irradiated by green LEDs, and the time-course changes in bilirubin photoisomers were measured by high-performance liquid chromatography. The cyclobilirubin production rates in humans, pigs, and monkeys were significantly higher than those in other species. The rate constant of (EZ)-cyclobilirubin production from (EZ)-bilirubin ‘k’ was significantly higher in humans and monkeys than in other species. In conclusion, bilirubin photochemical kinetics under green spectrum LEDs in humans were characterized by a high cyclobilirubin production rate at a low substrate concentration. The bilirubin photochemical kinetics in monkeys were similar to those in humans.
Highlights
Phototherapy using light-emitting diodes (LEDs) centered on the green spectrum, which has a high cyclobilirubin production rate, was as effective as that centered on the blue spectrum for neonatal hyperbilirubinemia
To develop a new light source centered on the green spectrum of 490–520 nm, it is important to evaluate the efficacy of phototherapy in humans for bilirubin reduction due to photochemical reactions, including
In this study, regarding the photochemical reaction when irradiating bilirubin bound to albumin with a light source centered on the green spectrum, the cyclobilirubin production rate differed among the animal species and was high in human serum albumin (SA), pig SA, and monkey serum
Summary
Phototherapy using light-emitting diodes (LEDs) centered on the green spectrum, which has a high cyclobilirubin production rate, was as effective as that centered on the blue spectrum for neonatal hyperbilirubinemia. This report describes the characteristic photochemical kinetics of bilirubin under green-spectrum LEDs in human, rat, rabbit, dog, pig, sheep, bovine and chicken serum albumin and rhesus monkey serum. To develop a new light source centered on the green spectrum of 490–520 nm, it is important to evaluate the efficacy of phototherapy in humans for bilirubin reduction due to photochemical reactions, including. To develop new light sources for phototherapy using animal models for human neonatal hyperbilirubinemia, it is necessary to clarify the differences in bilirubin photochemical reactions, including cyclobilirubin production, among animal species under both green and blue light sources. As the photochemical reactions of bilirubin are due to differences in albumin, we investigated these photochemical reactions, including cyclobilirubin production, in humans and several animal species
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